Development of Integrated Atomic Force Microscopy and Fluorescence Microscopy for Single-Molecule Analysis in Living Cells
10.11895/j.issn.0253-3820.171313
- VernacularTitle:原子力显微镜-荧光显微镜联用技术在活细胞单分子检测中的应用
- Author:
Ge Ge QIN
1
;
Hui Wen LI
;
Chao Jia XU
;
Long Xiao KOU
;
Rong ZHAO
;
Fang LUO
;
Hong Xiao FANG
Author Information
1. 中国科学院化学研究所
- Keywords:
Atomic force microscopy;
Fluorescence microscopy;
Single-molecule fluorescence imaging;
Single-molecule force spectroscopy;
Single-molecule analysis;
Review
- From:
Chinese Journal of Analytical Chemistry
2017;45(12):1813-1823
- CountryChina
- Language:Chinese
-
Abstract:
Atomic force microscope ( AFM) and fluorescence microscope ( FM) have been emerging as two most commonly used tools for single-molecule study in living cells. Combining the advantages of two microscopes, the development of the integrated AFM-FM technique with high spatiotemporal resolution and multi-function has attracted increasing interest. In this review, the principles of AFM single-molecular force spectroscopy and single-molecule fluorescence imaging were briefly discussed, and the recent advances in the integrated AFM-FM instrumentation were summarized. Subsequently based on our own research in the investigation of ligand-receptors interactions with the integrated AFM-FM technique, its applications in live-cell single-molecule imaging and characterization were introduced.
