An Electrochemiluminescent Method to Detect A Dipeptide Related to Diabetes
10.11895/j.issn.0253-3820.171284
- VernacularTitle:电化学发光分析法测定糖尿病相关二肽
- Author:
Chen FANG
1
;
Juan Li ZHENG
;
Yan Su ZHAI
;
Feng Yi TU
Author Information
1. 苏州大学材料与化学化工学部
- Keywords:
Electrochemiluminescence;
Luminol;
Diabetes;
Glucagon-like peptide 1;
Dipeptide;
Dipeptidyl peptidase IV
- From:
Chinese Journal of Analytical Chemistry
2017;45(12):1951-1955
- CountryChina
- Language:Chinese
-
Abstract:
In this work, a simple and fast approach for dipeptide detection was developed. AuNPs were decorated onto the surface of indium tin oxide ( ITO ) glass to serve as the working electrode to trigger the electrochemiluminescence ( ECL) of luminol. The property of this electrode was characterized by transmission electron microscopy ( TEM ) , scanning electron microscopy ( SEM ) , electrochemistry and spectroscopic method. Under the optimum conditions, the dipeptide His-Ala could be detected within a linear range from 2. 44×10-11 mol/L to 1. 22×10-7 mol/L, with a detection limit of 2. 42×10-12 mol/L (S/N=3). In human body, the glucagon-like peptide 1(GLP-1) might lose activity during degradation under the enzymatic action of dipeptidyl peptidase IV (PDD-IV), meanwhile release same concentrated His-Ala dipeptide. Thus, the detection of His-Ala dipeptide was of great significance for investigation of diabetes not only for understanding the relation between GLP-1, PDD-IV and its inhibitor, but also the drug discovering because of its potential availability as the target to control the blood GLP-1 level of type II diabetics.
