Optimization of Extraction Technology for Flavonoids in Leaves and Seeds of Hippophae rhamnoides by Multiindex-Orthogonal Test
10.6039/j.issn.1001-0408.2017.34.27
- VernacularTitle:多指标-正交试验优化沙棘叶和沙棘籽中黄酮的提取工艺
- Author:
Renjie HUI
1
;
Jing FENG
;
Mohan LIN
;
Bainian FENG
Author Information
1. 江南大学药学院
- Keywords:
Leaves of Hippophae rhamnoides;
Seeds of Hippophae rhamnoides;
Orthogonal test;
Extraction technology;
Fla-vonoid aglycone
- From:
China Pharmacy
2017;28(34):4856-4859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology for flavonoids in leaves and seeds of Hippophae rhamnoides. METHODS:Using the total extraction rate of 6 flavonoid aglycones(catechins,rutin,myricetin,quercetin,kaempferol,isorham-netin)as index,ethanol volume fraction,extraction time,extraction times,material-lipid ratio as investigation indexes,L9(34)or-thogonal test was designed to optimize the extraction technology of flavonoids in leaves and seeds of H. rhamnoides,and verifica-tion test was carried out. RESULTS:The optimum extraction technology for flavonoids in leaves of H. rhamnoides was ethanol vol-ume fraction of 70%,extracting for 3 times with material-lipid ratio of 1:16,and 2.0 h each time;and that of seeds was ethanol volume fraction of 50%,extracting for 3 times with material-lipid ratio of 1:24,and 1.5 h each time. In verification test,the total extraction rate of 6 flavonoid aglycones was 56.4 mg/g in the leaves (RSD=1.4%,n=3) and 15.4 mg/g in the seeds (RSD=3.4%,n=3). CONCLUSIONS:Optimized extraction technology is simple,stable,feasible,and can be used for extracting the fla-vonoids in leaves and seeds of H. rhamnoides.
