Apoptosis of HepG-2 cells induced by total flavonoids of Verbena officinalis L. and possible mechanism
10.13220/j.cnki.jipr.2017.08.008
- VernacularTitle:马鞭草总黄酮诱导肝癌HepG-2细胞凋亡及可能机制
- Author:
jia Xian LI
1
;
ping Li REN
;
ju Shao JING
Author Information
1. 漯河医学高等专科学校生化教研室
- Keywords:
human hepatocellular carcinoma;
HepG-2;
Verbena officinalis L.;
Reactive oxygen species(ROS);
membrane potential;
Caspase-3;
Caspase-8;
Caspase-9;
P53;
cell apoptosis
- From:
Journal of International Pharmaceutical Research
2017;44(8):790-794
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the apoptosis and mechanisms of HepG-2 cells induced by total flavonoids of Verbena offici?nalis L.(TFV). Methods HepG-2 cells were cultured with different concentrations of TFV. The apoptosis of HepG-2 cells was evalu?ated by flow cytometry and DNA ladder. Reactive oxygen species(ROS)and membrane potential were measured by flow cytometry. Ex?pression of Caspase-3,Caspase-8,Caspase-9,and P53 was analyzed by Western blotting. Results TFV 50,100 and 200 mg/L in?creased the apoptosis rate(P<0.01),increased ROS levels of HepG-2 cells(P<0.01),and decreased the mitochondria membrane potential(P<0.05,P<0.01). TFV(50,100 and 200 mg/L)increased the proteins′ expression of Caspase-3,Caspase-9 and P53 (P<0.05,P<0.01). Conclusion TFV may induce the apoptosis of HepG-2 cells via increasing ROS levels,decreasing mitochon?dria membrane potential,and up-regulateing the expression of Caspase-3,Caspase-9 and P53 protein in HepG-2 cells.
