Simutaneous determination of ursolic acid and oleanolic acid in Ziziphora clinopodioides Lam. by in situ pretreatment-thin layer chromatography scanning
10.13220/j.cnki.jipr.2017.08.012
- VernacularTitle:原位预处理-薄层扫描法同时测定唇香草中熊果酸和齐墩果酸的含量
- Author:
huan Wen DING
1
;
yin Tian YANG
;
jia Xue ZHANG
;
yan Hai XU
;
ying Xiao ZHOU
Author Information
1. 新疆医科大学中心实验室
- Keywords:
Z. clinopodioides;
thin layer chromatography scanning;
ursolic acid;
oleanolic acid
- From:
Journal of International Pharmaceutical Research
2017;44(8):812-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the simultaneous determination of ursolic acid(UA)and oleanolic acid(OA) in Ziziphora clinopodioides Lam.,and the quantitative determination the UA and OA contents in the different Z. clinopodioides plant samples collected with various parts of the plant at different times,from different regions of Xinjiang,China. Methods Dual wave?length scanning method was used for the quantification of UA and OA spots on a silica gel G plate in the TLC analysis. The samples loaded on the silica gel G plate were in situ treated with the 1%iodine solution in dichloromethane,and then the plate was developed using cyclohexane,cyclohexane-chloroform-ethyl acetate-formic acid(20:5:8:0.1)as the developing solvent. In the dual wavelength scanning,the measurement wavelength was 530 nm and the reference wavelength was 700 nm. Results The UA and OA spots in samples were well separated on the TLC plate and could be simultaneously quantified by the present method. The average contents of UA and OA in Z. clinopodioides plant samples from 18 different areas were(1.84 ± 0.41)and(2.82 ± 0.89)mg/g,respectively. The contents of UA and OA in the plant increased from late spring to early summer and then decreased thereafter. As to the different parts of the plant,the contents of UA and OA were highest in leaves and lowest in stems. Conclusion The method is simple,fast and accu?rate. The present results provided basic data for further evaluation of the quality of Z. clinopodioides resources.
