Fluorous Solid-Phase Extraction Assisted New Methods for Analysis of Biomolecules with Mass Spectrometry
10.11895/j.issn.0253-3820.171297
- VernacularTitle:氟固相萃取辅助的生物分子质谱分析新方法
- Author:
Cheng ZHANG
1
;
Yang Tian YAN
;
Chuan CHEN
;
Jie Hao LU
Author Information
1. 复旦大学化学系
- Keywords:
Fluorous solid-phase extraction;
Biological mass spectrometry;
Separation technique;
Proteomics;
Glycomics;
Review
- From:
Chinese Journal of Analytical Chemistry
2017;45(12):1857-1864
- CountryChina
- Language:Chinese
-
Abstract:
Fluorous solid-phase extraction ( FSPE ) is a solid-phase extraction technique based on fluorous affinity between perfluorous compounds. It requires derivatization on analytes with fluorous tags and further specific separation accomplished by perfluorinated solid phase. This technique has extended to various research fields with broad application including organic synthesize, catalysis, chemical and biological analysis. Recently, owing to its good compatibility with mass spectrometry, new analytic techniques relying on FSPE coupled to biological mass spectrometry have received wide attention. This review briefly introduced the principle of FSPE and emphasized on its application for the analysis of biomolecules with mass spectrometry, as well as its outlook of future development.
