Development of A Multiplex PCR Method for Detecting Six Types of Viruses Causing Encephalitis

VernacularTitle:6种常见脑炎病毒多重PCR检测体系的建立和应用
Author: nan Xiao WANG ¹ ; qi Shi LU ; qian Qian DUAN ; gang Jian JI ; jun Li ZHANG ; wan Wan LIU ; sheng Bang XI ; ping Wan SUN
Author Information

1. 苏州大学药学院药理学系
Keywords: multiple polymerase chain reaction; viral encephalitis; nucleicacid detection
From: Journal of Modern Laboratory Medicine 2017;32(6):11-14,18
CountryChina
Language:Chinese
Abstract: Objective To develop a new mPCR method for rapid diagnosis of six types of encephalitis causing viruses of HS-VI,HSVII,VZV,EBV,EV71 and CMV.Methods Six pairs of specific primers for CMV,EV71,HSV I,VZV,EBV and HSV II were designed.The mPCR detection method was established and the sensitivity was detected.In order to verify the clinical application value of their multiplex PCR system,fifteen cerebrospinal fluid specimens of clinically suspected VE from the First Affiliated Hospital of Soochow University from 2014 to 2015 were examined by the mPCR method.Results The 6 pairs of primers did not interfere with each other,and the sensitivity of the mPCR system was over 103copies/μl.Among 15 cerebrospinal fluid specimens from patients with suspected viral encephalitis,six specimens(6/15,40%)were tested positive by the mPCR.Among them,HSV I was 5 and CMV was 1.Conclusion The mPCR method for detecting six types of en-cephalitis-associated virus at same time was established with high specificity,sensitivity and stability.