Study on the Specificity Enrichment of Hepatoma Cell Monoclonal Antibody Against Hepatocellular Carcinoma and Gastric Cancer
10.3969/j.issn.1671-7414.2017.06.038
- VernacularTitle:肝癌细胞单克隆抗体磁珠对人肝癌、胃癌细胞株特异性富集的实验研究
- Author:
bin Wen HAO
1
;
fen Fen XIANG
;
hong Hong YUE
;
Yan FAN
;
Jian XU
;
li Qiao LIU
;
Rong WU
;
dong Xiang KANG
Author Information
1. 上海中医药大学附属普陀医院检验科
- Keywords:
4-E9;
epithelial adhesion molecules;
circulating tumor cells
- From:
Journal of Modern Laboratory Medicine
2017;32(6):132-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the combination of 4-E9 immunomagnetic beads and epithelial adhesion mole-cules(Epacm)beads can enhance the enrichment efficiency of MCF-7,BEL-7402 and BGC-823 cells.Methods A mono-clonal antibody was prepared and ligated with magnetic bead by a biotin and a streptavidin to prepare an immunomagnetic beads.The enrichment rate of MCF-7,BEL-7402 and BGC-823 cells was detected by the combination of two kinds of immu-nomagnetic beads and two kinds of immunomagnetic beads.Results The encapsulation rate of 4-E9 immunomagnetic beads was 57.8%,and the encapsulation rate of Epcam immunomagnetic beads was 65.8%.4-E9 immunomagnetic beads on MCF-7,BGC-823 and BEL-7402 cell capture rate was(44±5.33)%,(71±11.33)% and(78.3±8.46)% respectively.Epcam immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell capture rate was(55.5±8.67)%,(78.88±13.11)% and (84.31±6.83)% respectively.The combination of two kinds of immunomagnetic beads on BGC-823,BEL-7402 and MCF-7cell capture rate was(80.4±8.33)%,(85.125±6.77)% and(93.23±4.33)% respectively.Joint beads group compared with 4-E9 immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell enrichment rate were statistically significant(P=0.03,0.03,0.04),and joint beads group compared with 4-E9 immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell enrichment rate were statistically signific(P=0.04,0.03,0.04).Conclusion The combination of two kinds of immunomag-netic beads can significantly improve the enrichment efficiency of Epcam immunomagnetic beads on BEL-7402,BGC-823 and MCF-7 cells.4-E9 antibody enrichment of circulating tumor cells may have some clinical value.
