Comparison of different laboratory detection methods of Clostridium difficile
10.3969/j.issn.1673-4130.2017.22.011
- VernacularTitle:艰难梭菌实验室不同检测方法的比较
- Author:
Min LI
1
;
Gang WANN
;
Xiaoliang MA
;
Xinmin XU
;
Huizhu WANG
;
Wenhao HUA
Author Information
1. 首都医科大学附属北京地坛医院检验科
- Keywords:
clostridium difficile;
enzyme linked immunosorbent assay;
antigen;
toxin;
identification medium
- From:
International Journal of Laboratory Medicine
2017;38(22):3102-3103,3106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the clinical feasibility of different methods for detecting Clostridium difficile (CD) .Methods The stool samples were collected from the patients with suspected antibiotic-related diarrhea during 2016 ,and the enzyme linked immunosorbent assay (ELISA) and identification medium culture method were used for detection .Then the sensitivity ,specificity and consistency were compared between the two methods .Results 29 cases of antigen-positive specimens were measured by ELISA ,including 25 cases of toxin-positive and 4 cases of toxin negative ;29 suspected strains were cultured by the selective identification medium ,which were identified by the mass spectrometry as 28 strains of Clostridium difficile and 1 strain of undetected species ;the sensitivity and specificity of ELISA method and identification culture medium method were 97% ,100% and 93% ,95% respectively ,both methods showed extremely good consistency (Kappa=0 .92) .Conclusion The ELISA method has the characteristics of fastness ,high efficiency ,time saving ,simple operation ,easy interpretation and so on ,and can rapidly and accurately screen CD related diarrhea diseases ;the medium culture method has a good specificity and can rapidly obtain the infectious strain ;their combined use can greatly increase the CD detection rate and has great help for late treatment .
