MiR-375 suppresses angiogenesis of hepatocellular carcinoma by targeting platelet-de-rived growth factor-C
10.3969/j.issn.1000-8179.2017.20.689
- VernacularTitle:miR-375调控PDGFC基因表达抑制肝癌血管生成
- Author:
WANG TAO
1
;
LI DONG
;
PENG JINGJING
;
LIU YU
;
SUN FEIFAN
;
WANG DAN
;
CHENG PENG
;
SU XIAOMEI
;
ZHANG LIN
Author Information
1. 中国人民解放军成都军区总医院肿瘤诊治中心 成都市610083
- Keywords:
primary hepatocellular carcinoma;
miR-375;
angiogenesis;
PDGFC
- From:
Chinese Journal of Clinical Oncology
2017;44(20):1007-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Abnormal angiogenesis is an important hallmark of HCC. Ectopic miR-375 overexpression led to repression of proliferation, migration, invasion, and colony formation, and it induced apoptosis in hepatoma cells as well. In this study, we explored the effect of miR-375 on HCC angiogenesis. Methods:We evaluated the antiangiogenic effects of miR-375 using human umbilical vein endothelial cells, tube formation assays, rat aortic ring sprouting assays, and chicken chorioallantoic membrane assays. Bioinformatics software was used to predict the downstream target gene of miR-375. MiR-375 regulation to target genes was explored by overexpres-sion and knockdown of miR-375 in hepatoma cells. Luciferase assay was performed to confirm its molecular mechanism. Rescue assay of target gene was further used to prove that miR-375 inhibited HCC angiogenesis by directly regulating its target gene. Results:MiR-375 inhibited HCC angiogenesis. Platelet-derived growth factor-C (PDGFC) was a potential target gene of miR-375. MiR-375 inhibited PDGFC expression in hepatoma cells by targeting its 3′-UTR. MiR-375 exerted its antiangiogenic effect partially by PDGFC inhibition. Conclusion:MiR-375 repressed tumor angiogenesis by targeting PDGFC in HCC.
