Comprehensive monitoring of charge analysis and research of active pharmaceutical ingredients in Shedan-Chenpi tablet by high performance liquid chromatography-mass spectrometry
10.3760/cma.j.issn.1673-4246.2017.12.014
- VernacularTitle:应用高效液相色谱-质谱法监测蛇胆陈皮片原料药的投料分析研究
- Author:
Minfeng ZHU
1
;
Xiaolin WANG
;
Hualing LUO
;
Xiang FENG
;
Maohua YUAN
;
Xiaohu LI
;
Fudong ZHANG
Author Information
1. 614000,四川省乐山市食品药品检验检测中心
- Keywords:
Hesperidin;
Shedan-Chenpi tablet;
Aurocholic acid sodium;
high performance Liquid chromatography-mass spectrometry
- From:
International Journal of Traditional Chinese Medicine
2017;39(12):1113-1117
- CountryChina
- Language:Chinese
-
Abstract:
Objective The monitoring of the feeding situation of active pharmaceutical ingredient in Shendan-Chenpi tablet by HPLC-MS/MS,and the content of sodium taurocholate and hesperidin.Methods Using ZORBAX Eclipse Plus C18(4.6 mm×250 mm, 5 μm)Column temperature 40 ℃, Mobile phase with acetonitrile-10 mmol/sodium acetate solution, using gradient elution program. Active ingredients were separated by HPLC, and the Electrospray Ionization Mass (ESI) source was applied and operated in the negative ion mode, and reactions ion monitoring mode(MRM) for quantitative analysis were selected. Results The proprietary Chinese medicine is judged by the prescription feeding process, through analysis and contrast of the medicinal materials, reference substance of primary mass spectrogram, secondary mass spectrogram of peak. The aurocholic acid sodium and hesperidin had a good linear relationship in 0.242×10-2-1.45×10-2μg(r=0.996 0), 0.688×10-2-10.30×10-2μg(r=0.999 2), and the precision test were 0.78% and 1.56%, and the recovery rate were 102%-103%,96%-109%. Conclusions The method was simple, accurate and reliable, high sensitive and fast. The comprehensive monitoring was applied to the Shedan-Chenpi tablet in feeding analysis and quality.
