Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism
10.11665/j.issn.1000-5048.20170517
- VernacularTitle:丝素肽对Aβ25-35致SH-SY5Y细胞损伤的保护作用及机制研究
- Author:
Haiyang YU
1
;
Song CHEN
;
Zheng XU
;
Xiangdong GAO
;
Wenbing YAO
Author Information
1. 中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室
- Keywords:
fibroin peptide;
Alzheimer' s disease;
neuroprotective effect;
Tau phosphorylation;
reactive oxy gen species
- From:
Journal of China Pharmaceutical University
2017;48(5):609-613
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at exploring the molecular mechanism of fibroin peptide in preventing the Aβ25-35-induced neuronal damages in SH-SY5Y cells.MTT was used to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced injuries in SH-SY5Y cells;Western blot was employed to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced hyperphosphorylation of Tau in SH-SY5Y cells;DCFH-DA probe method was used to detect the effect of fibroin peptide on the Aβ-induced production of intracellular reactive oxygen species (ROS) in SH-SY5Y cells.The results indicated that fibroin peptide could improve the activity of the PP2A and inhibit the activity of GSK-3β to decrease the hyperphosphorylation of Tau induced by Aβ25-35.Fibroin peptide could significantly prevent the Aβ25-35-induced neuronal damages and multisite Tau hyperphosphorylation.In addition,fibroin peptide could also reduce oxidative damage to protect neurons by significantly decreasing the Aβ25-35-induced production of intracellular ROS.
