miRNA-101-3 p inhibits proliferation and migration of gastric cancer cells by targeting EZH2
10.3969/j.issn.1000-4718.2017.12.006
- VernacularTitle:miRNA-101-3p靶向调控EZH2蛋白抑制 胃癌细胞增殖和迁移
- Author:
tian Xiao ZHANG
1
;
yan Xiao ZHANG
;
ya Sai HUANG
;
Qian WANG
;
Wei LIU
Author Information
1. 山西医科大学汾阳学院医学检验系
- Keywords:
Gastric cancer;
MicroRNA;
Cell proliferation;
Cell migration;
Apotosis;
Enhancer of zeste hom-olog 2
- From:
Chinese Journal of Pathophysiology
2017;33(12):2143-2150
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of microRNA-101-3p (miRNA-101-3p) on the proliferation, apopto-sis and invasion of gastric cancer cells and the possible regulatory mechanisms .METHODS: The expression of miRNA-101-3p in two kinds of gastric cancer cells and a gastric mucosal cell line was detected by real -time PCR.The miRNA-101-3p was overexpressed by Lipofectamine 2000 transfection with miRNA-101-3p mimics.The effects of miRNA-101-3p on cell cycle distribution and apoptosis were analyzed by flow cytometry .The effects of miRNA-101-3p on cell proliferation and migration abilities were detected by CCK-8 assay, trypan blue exclusion test and Transwell assay .The protein expression of enhancer of zeste homolog 2 ( EZH2) was determined by Western blot .RESULTS: The expression of miRNA-101-3p in gastric cancer cells was lower than that in gastric mucosal cells (P<0.05).The gastric cancer cell MGC-803 had the low-est expression level of miRNA-101-3p.The result of flow cytometry showed that the population of S phase was reduced , and the population of G0/G1 phase and the early stage apoptotic rate were increased after the expression of miRNA-101-3p was overexpressed (P<0.05).The results of CCK-8 assay, trypan blue exclusion test and Transwell assay showed that overex-pression of miRNA-101-3p significantly reduced the proliferation and migration abilities of gastric cancer cells (P<0.05). Overexpression of miRNA-101-3p decreased the protein level of EZH2 (P<0.05).CONCLUSION:miRNA-101-3p may suppresses the gastric cancer cell proliferation and migration , and promotes the gastric cancer cell apotosis by down-regula-tion of EZH2.
