Effects of S100 B, AQP4 and CX43 expressions on the function of the blood brain barrier in hippocampus of diabetic rats with depression
10.3969/j.issn.1001-6325.2017.12.002
- VernacularTitle:S100B、AQP4和CX43表达紊乱对糖尿病并发抑郁症大鼠海马血脑屏障功能的影响
- Author:
shan Yuan HAN
1
;
Qing DU
;
qing Hong ZHAO
;
hong Yu WANG
;
Hui YANG
;
Pan MENG
;
hui Yu QIN
Author Information
1. 湖南中医药大学第一附属医院中心实验室
- Keywords:
diabetes mellitus with depression;
blood-brain barrie;
S100B;
aquaporin 4;
connexin 43
- From:
Basic & Clinical Medicine
2017;37(12):1663-1667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of S 100B, AQP4, and CX43 on the function of the blood brain barrier in the hippocampus of diabetic rats with depression .Methods Rats were divided into the diabetic group [ two weeks of high-fat diet and injection of Streptozotocin (STZ, 38 mg/kg)], depression group(chronic unpredictable stress for 28 days) , the diabetes mellitus with depression group ( combined with the above two methods ) , and the control group .The behavior of rats was evaluated with open-field test and Morris test .The expressions of AQP 4 and CX43 in rat hippocampus blood-brain barrier were detected by immunocytochemistry .Serum S100B level was detected by ELISA.Results Compared with control group, the number of autonomic activity and the space exploration times were decreased, the escape latencies were significantly longer in the Morris water maze test(P<0.05 or P<0.01) of the diabetes group and depression group; Serum S100B levels increased significantly ( P<0.01);the expressions of AQP4 and CX43 were decreased(P<0.01).Compared with diabetic group, the number of auto-nomic activity and the space exploration times were decreased , the escape latencies were significantly longer in the Morris water maze test( P<0.05 or P<0.01) of the diabetes group and depression group;Serum S 100B lev-els increased significantly( P<0.05);the expressions of AQP4 and CX43 were decreased(P<0.05 or P<0.01). Conclusions S100B, AQP4 and CX43 expression disorder may be one of the mechanisms of diabetes mellitus complicated with depression.
