Effect of potassium iodate on the cell cycle of human thyroid cancer cells
10.3969/j.issn.1001-6325.2017.12.012
- VernacularTitle:碘酸钾对人甲状腺癌细胞系周期的影响
- Author:
Hong LI
1
;
yue Jia ZHANG
;
ming Song LI
;
li Jin LENG
;
Rui LI
;
Hui HE
;
xiong Fei PANG
Author Information
1. 解放军303医院普外科
- Keywords:
thyroid cancer;
potassium iodate;
cyclin D1;
proliferating cell nuclear antigen Ki67
- From:
Basic & Clinical Medicine
2017;37(12):1724-1728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of potassium iodate (KIO3) on the proliferation, cell cycle, and the mRNA/protein levels of cyclin D 1 and Ki67 of SW579 cells, a human thyroid squamous carcinoma cell line . Methods The effects of different doses of KIO3(0,10-6,10-5,10-4,10-3,10-2,10-1 mol/L)on SW579 cell prolifer-ation were assessed by CCK8 method.SW579 cells were then treated with 0 (control), 10-6 or 10-2 mol/L KIO3 for 48 h.The cell cycle was measured by flow cytometry .The mRNA and protein levels of cyclinD 1 and Ki67 were re-spectivelyanalyzedbyreal-timePCRandWesternblot.Results 10-6and10-2mol/LofKIO3respectivelyexhibi-ted the most promoting and suppressive effect on SW579 cell proliferation.G1 phase of cells in 10-6 group short-ened significantly( P<0.05) , and S phase prolonged significantly( P<0.05); while each phase of cells in 10-2 mol/L group changed in the opposite way( P<0.05) .KIO3 at the dose of 10-6 mol/L significantly up-regulated the mRNA levels of cyclin D1 and ki67 in cells( P<0.05) ;whereas, the mRNA and protein levels of cyclin D1 and Ki67 were significantly down-regulated in cells treated with 10-2 mol/L KIO3( P<0.05) .Conclusions Different doses of KIO3 affect the proliferation and cell cycle of SW579 cells probably by regulating the levels of cyclin D1and Ki67 .
