The effect of MK gene overexpression in hepatic carcinoma cells on multidrug resistance
10.3760/cma.j.issn.1007-631X.2017.11.018
- VernacularTitle:中期因子过表达对肝癌细胞多药耐药的影响
- Author:
Huaping SHEN
1
;
Shiyu ZHOU
;
Jiewei XU
;
Jian QIU
;
Yunhai WEI
;
Mingjie ZHANG
;
Guolei ZHANG
;
Qiang YAN
Author Information
1. 湖州市中心医院普外科
- Keywords:
Carcinoma,hepatocellular;
Multidrug resistance-associated proteins;
Midkine
- From:
Chinese Journal of General Surgery
2017;32(11):962-965
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of enhanced MK gene expression in hepatic carcinoma cells.Methods The recombinant plasmid pIRES2-EGFP-MK was transfected into SMMC 7721 cells.The mRNA and protein expression levels of MK gene in these cells were determined by real-time PCR,Western blotting and flow cytometry.The intracellular DNR accumulation of these cells was measured by flow cytometry.To investigate the effect of MK gene mediated multidrug resistance,MTT assay was employed to determine the cellular sensitivity of different chemotherapeutic drugs in MK-overexpressed SMMC 7721 cells.Results The mRNA and protein expression levels of MK gene significantly increased after the recombinant plasmid pIRES2-EGFP-MK transfected into SMMC 7721 cells,suggesting that the recombinant plasmid pIRES2-EGFP-MK can enhance the transcription of MK effectively.The DNR accumulation of MK transfected cells decreased significantly (4.06 ± 0.88,P ＜ 0.05),and IC50 of MK transfected cells to ADM/5-FU increased significantly (15 ± 3,27 ± 4,P ＜ 0.05).Conclusions After the recombinant plasmid pIRES2-EGFP-MK transfected into hepatic carcinoma cells,expression of midkine increased,enhancing the resistance of hepatic carcinoma cells to chemotherapeutic drugs.
