Repression effect of metformin on photo-damage of human skin fibroblasts induced by UVA
10.3969/j.issn.1001-6325.2017.12.016
- VernacularTitle:二甲双胍抑制UVA诱导的人皮肤成纤维细胞的光老化效应
- Author:
Yi LIN
1
;
li Xiao ZHOU
;
yun Hai CHEN
;
li Chun ZHANG
Author Information
1. 杭州外国语学校
- Keywords:
metformin;
UVA;
human skin fibroblasts;
photo-damage
- From:
Basic & Clinical Medicine
2017;37(12):1741-1745
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the inhibitory effect and mechanism of metformin ( MF) on photo-damage of human skin fibroblasts ( HSF) induced by UVA .Methods Human skin fibroblasts were randomly divided into con-trol group, UVA group and UVA+MF group.The proliferation of HSF was detected by CCK-8 assay kit.SA-β-gal staining was performed to evaluate the senescence state .The level of ROS was examined by fluorescence probe DCF-DA staining using flow cytometry .Real-time PCR was used to determine mRNA expression of senescence -asso-ciated signals of MMP 1 and MMP3.The protein expression of MMP 1, MMP3, SOD1 and SOD2 were measured by Western blot .R esults To the proliferation of HSF , 0.01 mmol/L Metformin had no significant effect , but 0.1 and 1 mmol/L Metformin depressed significantly ( P<0.05 ) .Compared with the Control group , it showed that UVA irradiation increased the positive rate of SA-β-gal staining ( P<0.01 ) , the level of ROS ( P<0.05 ) , mRNA and protein expression of MMP1 and MMP3 significantly(P<0.01);Also decreased the expression of SOD1 and SOD2 ( P<0.01) .Compared with the UVA group , it showed that metformin decreased the positive rate of SA-β-gal staining (P<0.05), the level of ROS(P<0.05), mRNA and protein expression of MMP1 and MMP3 significantly(P<0.05);Also increased the expression of SOD 1 ( P<0.01 ) and SOD2.Conclusions Metfomin can inhibit photo-damage of human skin fibroblasts induced by UVA via decreasing ROS and metal matrix protease generation , also the improvement of cellular antioxidant capacity .
