Dynamic Expressions of Tenascin-C at Different Phases of Atherosclerosis in ApoE-/- Mice
10.3969/j.issn.1000-3614.2017.12.018
- VernacularTitle:腱糖蛋白-C在载脂蛋白E基因敲除小鼠动脉粥样硬化斑块不同时期表达的变化
- Author:
Yan LI
1
;
cheng Jia SONG
;
lang Hai YAN
;
wen Jun HUANG
;
Yu LU
;
long Zhan MA
;
bin Hai SHI
Author Information
1. 南京医科大学第一附属医院 放射科
- Keywords:
Atherosclerosis;
Apolipoprotein E;
Mice;
knockout;
Tenascin
- From:
Chinese Circulation Journal
2017;32(12):1217-1221
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the dynamic expressions of tenascin-C (TN-C) at different phases of atherosclerosis in ApoE-/-mice. Methods: Our research included in 2 groups: ApoE-/- group, n=50 SPF male mice with ApoE-/- and Control group, n=50 wild male C57BL/6 mice. Atherosclerosis model was established by high fat diet in both groups. The mice were sacrificed at 4, 8, 16, 24 and 32 weeks, blood lipids were examined, pathologic changes of plaque were observed by microscope for quantitative analysis and TN-C expressions in atherosclerosis plaque were measured by immunohistochemistry. Results: Compared with Control group, ApoE-/- group had elevated blood levels of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C), both P<0.05. In ApoE-/- group, plaque area and the ratio of plaque area/lumen area were increasing upon prolonged modeling time, all P<0.05; TN-C expressions were increasing by progress of atherosclerosis, the highest TN-C expression was found at 32 weeks of modeling (0.49±0.07) which was higher than it was at 8 weeks (0.04±0.02), 16 weeks (0.12±0.03) and 24 weeks (0.21±0.04), all P<0.05. Conclusion: TN-C expression was increasing with plaque progress which might be related to the development of atherosclerosis and plaque instability.
