Effects of simvastatin on expression of Bcl-2 and Bax in myocardium of rats with renal ischemia-reperfusion injury
10.3969/j.issn.1000-4718.2017.12.026
- VernacularTitle:辛伐他汀对大鼠肾缺血再灌注损伤后心肌 Bcl-2和Bax蛋白表达的影响
- Author:
Jiao JING
1
;
ling Hai MA
;
sheng Wen YAN
;
zhong Yong ZHANG
;
qing Yu ZHANG
;
wei Zong JIAO
Author Information
1. 石家庄医学高等专科学校
- Keywords:
Simvastatin;
Renal ischemia-reperfusion injury;
Myocardium;
Malondialdehyde;
Superoxide dis-mutase;
Lactate dehydrogenase
- From:
Chinese Journal of Pathophysiology
2017;33(12):2274-2277
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of simvastatin on myocardial tissue after renal ischemia-reperfusion in-jury and its mechanism .METHODS:A rat model of renal ischemia-reperfusion injury was prepared by clamping the bilat-eral renal arteries for 45 min.The rats (n=36) were randomly divided into sham operation group , renal ischemia-reperfu-sion (I/R) group and simvastatin group with 12 rats in each group.The content of serum creatinine (SCr), blood urea ni-trogen ( BUN) and myocardial tissue malondialdehyde ( MDA) , the myocardial activity of lactate dehydrogenase ( LDH) , creatine kinase (CK) and superoxide dismutase (SOD), and the myocardial protein expression of Bcl-2 and Bax were de-tected.RESULTS:Compared with sham operation group , the content of SCr , BUN and myocardial MDA , and the myo-cardial activity of LDH and CK in I/R group were significantly increased (P<0.05), and the activity of SOD was signifi-cantly decreased (P<0.05).Compared with I/R group, the content of SCr, BUN and myocardial MDA, and the myocar-dial activity of LDH and CK in simvastatin group were significantly decreased ( P<0.05 ) , while SOD activity was en-hanced (P<0.05).The protein expression of Bcl-2 and Bax in sham operation group was less than that in I/R group (P<0.05), and the protein level of Bax in simvastatin group was significantly lower than that in I /R group (P<0.05), while the protein level of Bcl-2 was increased (P<0.05).CONCLUSION:Simvastatin has a protective effect on the my-ocardium of the rats with renal ischemia-reperfusion injury , and the protective mechanism may be related to the elimination of free radicals by simvastatin , increase in the protein expression of Bcl-2 and decrease in the protein expression of Bax .
