Effects of NF-κB inhibitor on nerve injury in rats after intracerebral hemorrhage
10.3969/j.issn.1000-4718.2017.12.027
- VernacularTitle:NF-κB抑制制对脑出血大鼠神经 损伤的影响
- Author:
Lu FENG
1
;
hua Yue XUE
;
bao Zheng XU
;
you Xiu YAN
;
ren Li FANG
Author Information
1. 台州市中心医院神经外科
- Keywords:
Nuclear factor-κB inhibitor;
Apoptosis;
Intracerebral hemorrhage;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2017;33(12):2278-2282
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the regulatory effect of nuclear factor-κB (NF-κB) inhibitor, pyrrolidine di-thiocarbamate (PDTC), on nerve function and neural cell apoptosis in rats after intracerebral hemorrhage (ICH).METH-ODS:SPF Sprague-Dawley rats were randomly divided into 4 groups with 6 rats in each group: sham group, ICH group, PDTC at low concentration (Plow) group and PDTC at high concentration (Phigh) group.Autologous blood injection was used to establish ICH model.After 2 h of surgery, the rats in Plow group and Phigh group were intraperitoneal injected with PDTC at 100 mg/kg and 200 mg/kg, respectively , while rats in sham group and ICH group were injected with the same volume of saline .The neurological function score was classified with modified Longa grading method .TUNEL assay was used to detected the neural cell apoptosis , and the content of malondialdehyde ( MDA) and the activity of superoxide dis-mutase (SOD) were measured.Furthermore, the protein levels of p-P65 and cleaved caspase-3 in brain tissues were deter-mined by Western blot .RESULTS: Compared with sham group , the rats in ICH group had higher neurological function score (P<0.05).After treatment with PDTC, the neurological function score was decreased (P<0.05), but no signifi-cant difference between P low group and P high group was observed .Compared with sham group , the number of apoptotic cells in ICH group was increased ( P<0.05 ) .After treatment with PDTC , the neural cell apoptosis was restrained , and the number of apoptotic cells in Phigh group was lower than that in Plow group (P<0.05).Compared with sham group, the con-tent of MDA was increased and the activity of SOD was decreased in ICH group (P<0.05).After treatment with PDTC, the content of MDA was decreased while the activity of SOD was increased , and the variation trend was more obvious in Phigh group (P<0.05).Compared with sham group, the protein levels of p-P65 and cleaved caspase-3 in ICH group were increased (P<0.05).After treatment with PDTC, the protein levels of p-P65 and cleaved caspase-3 were decreased, and those in Phigh group were lower than those in P low group.CONCLUSION: NF-κB inhibitor PDTC plays a role in the se-condary brain injury after ICH , and the protective effect increases at the higher dose .The mechanism may be related to re-ducing MDA content and increasing SOD activity , and further inhibiting neural cell apoptosis .
