Profiling the dynamic changes of PBMC immune-related indicators in patients with lung cancer after chemotherapy cycles
10.3969/j.issn.1000-8179.2017.22.642
- VernacularTitle:初诊肺癌患者多次化疗外周血免疫相关指标动态变化的研究
- Author:
YOU DUO
1
,
2
;
新乡医学院第一附属医院肿瘤二科
;
CHEN YANAN
;
YANG RU
;
XU ZHISHAN
;
LU PING
;
ZHONG GENSHEN
Author Information
1. 新乡医学院第一附属医院生物治疗实验室(河南省卫辉市453100)
2. 新乡医学院第一附属医院肿瘤二科
- Keywords:
immunotherapy;
T lymphocyte surface co-signaling molecules;
lung cancer;
multi-cycle chemotherapy
- From:
Chinese Journal of Clinical Oncology
2017;44(22):1135-1140
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the optimal time point for combining chemotherapy and immunotherapy and provide an experimen-tal basis for immunotherapy intervention in clinical. Methods:Twenty-three lung cancer patients who completed five chemotherapy cycles between November 2015 and December 2016 in the First Affiliated Hospital of Xinxiang Medical University were enrolled in this study. Numbers of T lymphocyte subsets, B lymphocytes, and NK lymphocytes in peripheral blood were counted. Expression levels of T lymphocyte co-suppression molecule and cytokines in the peripheral blood mononuclear cell were detected using flow cytometry to analyze the dynamic changes of such indicators from one cycle to five cycles of chemotherapy. Results:Significant decreases in the lev-els of CD8+T lymphocytes, CD19+B lymphocytes, and CD16+CD56+NK cells and an increase in CD4+T lymphocytes were observed in the course of multi-cycle chemotherapy for patients with lung cancer. Differences were statistically significant (P<0.05). The co-suppres-sion molecular expression of PD-1, CTLA-4, and CCR-4 with T lymphocytes was downregulated, and the differences were significant (P<0.05). Conclusion:Profiling the dynamic changes of lymphocyte subsets and the expression of T lymphocyte co-suppression molecule are significant in multiple chemotherapy cycles for patients with lung cancer. In the later stage, the combined application of PD-1, PD-L1, CTLA-4, or CCR-4 antibody may exert good therapeutic effects for patients with a high expression level of related immune check-points.
