Neuroprotective effect of fasudil combined with bone marrow-derived neural stem cells on mice with experimental autoimmune encephalomyelitis
10.3969/j.issn.1000-4718.2017.12.001
- VernacularTitle:Fasudil联合骨髓源神经干细胞对EAE小鼠的 神经保护作用
- Author:
bin Guo SONG
1
;
ping Guo XI
;
hua Yan LI
;
shan Jia LI
;
chun Jian LIU
;
Zhi CHAI
;
guo Bao XIAO
;
xian Guang ZHANG
;
gen Cun MA
Author Information
1. 山西大同大学脑科学研究所
- Keywords:
Fasudil;
Bone marrow-derived neural stem cells;
Experimental autoimmune encephalomyelitis;
Neurotrophic factors
- From:
Chinese Journal of Pathophysiology
2017;33(12):2113-2120
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the neuroprotective effect of fasudil combined with bone marrow -derived neural stem cells ( BM-NSCs) on the mice with experimental autoimmune encephalomyelitis ( EAE).METHODS: Female C57BL/6 mice (8~10 weeks old, n=32) were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) to establish chronic EAE model .The mice were randomly divided into control ( ddH2 O ) group, fasudil group , BM-NSCs group , and fasudil+BM-NSCs group .The clinical score and body weight were recorded every other day .The expression of neurotrophic factors was determined by immunofluorescence staining .RESULTS:In comparison with ddH2O group, fasud-il combined with BM-NSCs delayed onset and ameliorated severity of EAE .The numbers of brain-derived neurotrophic fac-tor, glial cell-derived neurotrophic factor , nerve growth factor , neurotrophin-3 and ciliary neurotrophic factor positive cells in fasudil group, BM-NSCs group and fasudil +BM-NSCs group were all increased in various extents .In particularly, the expression of these neurotrophic factors in fasudil +BM-NSCs group was significantly higher than that in the mice treated with fasudil or BM-NSCs alone (P<0.01).CONCLUSION:Fasudil combined with BM-NSCs promotes the expression of neurotrophic factors and improves microenvironment of central nervous system , thus playing a positive role in neural restora-tion and regeneration through a synergistic and superimposed effect .
