Eeffect of HMGB1 on expression of NF-κB in BV-2 cells stimulated with Aβ25-35
10.3969/j.issn.1000-4718.2017.12.004
- VernacularTitle:高迁移率族蛋白1对Aβ25-35刺激下BV-2 细胞NF-κB表达的作用
- Author:
Yuan HAN
1
;
Ke NAN
;
fang Fang XIANG
;
juan Qian FANG
;
miao Chen HUANG
;
yuan Hui YONG
;
Hong CAO
;
Jun LI
Author Information
1. 温州医科大学附属第二医院育英儿童医院麻醉科
- Keywords:
High mobility group box-1 protein;
Amyloidβ-protein;
BV-2 cells;
Nuclear factor-κB;
RNA in-terference
- From:
Chinese Journal of Pathophysiology
2017;33(12):2134-2138
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of high mobility group box-1 protein (HMGB1) on the expression of nuclear factor-κB ( NF-κB) in BV-2 cells stimulated with amyloid β-protein ( Aβ) 25-35 .METHODS:Cultured BV-2 cells in logarithmic growth phase were divided into 4 groups:normal cell group ( without any treatment ) , model group ( treated with Aβ25-35 at 40 μmol/L) , RNA interference ( RNAi) group ( conducted with HMGB1-siRNA followed by Aβ25-35 stimula-tion) and solvent control group (treated with 0.1% DMSO).After treatment with Aβ25-35 for 24 h, the protein levels of HMGB1 and NF-κB in BV-2 cells were determined by Western blot .RESULTS:Aβ25-35 at 40μmol/L was used to stimu-late BV-2 cells.The GFP fluorescence-tagged HMGB1-siRNA (30 nmol/L) was used to transfect BV-2 cells and its trans-fection efficiency was about 80%~90%.The results of Western blot showed that the protein level of HMGB 1 was signifi-cantly decreased after the interference of siRNA fragment (P<0.05).The protein levels of HMGB1 and nucleic NF-κB p65 were dramatically increased in BV-2 cells stimulated with Aβ25-35(P<0.05).After RNA interference with HMGB1, the expression of HMGB1 and nucleic NF-κB p65 were significantly decreased in BV-2 cells stimulated with Aβ25-35 ( P<0. 05).CONCLUSION:RNA interference with HMGB1 reduces the expression of nucleic NF-κB in BV-2 cells stimulated with Aβ25-35 .
