Effect of icariine combined with embryonic stem cells on apoptosis of kidney epithelial cells in contrast-induced nephropathy rat models
10.13481/j.1671-587x.20170518
- VernacularTitle:淫羊藿苷联合胚胎干细胞对造影剂肾病大鼠模型肾上皮细胞凋亡的影响
- Author:
Yipa SAI
1
;
Yuanchun SONG
;
Jing LIU
;
Haiyan ZHAO
Author Information
1. 甘肃省人民医院儿科
- Keywords:
icariin;
embryonic stem cells;
contrast-induced nephropathy;
kidney epithelial cells;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2017;43(5):953-957
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of icariin combined with embryonic stem cells on the renal epithelial cells in the contrast-induced nephropathy (CIN) rats and its possible mechanism,and to provide experimental evidence for clinical application of icariin.Methods:Fifty SD rats were randomly divided into,control group,model group,icariin group,stem cell group and combination group (n =10).The rat models were established by tail vein injection of INDO,L-NAME and diatrizoate.The serum creatinine (Scr) levels of the rats were detected before and after modeling.The expressions of p38MAPK and caspase-3 mRNA and protein were detected by semi-quantitative RT-PCR and Western blotting method.The apoptotic rate of renal epithelial cells was detected by flow cytometry.Results:The levels of Scr of rats were significantly increased 48 h after combination contrast agent injection,suggesting that the CIN models were successfully set up.Compared with model group,the Scr level of the rats in group was significantly decreased (P＜0.05).compared with model group the expression levels of p38MAPK and caspase-3 mRNA and protein in icariin group and stem cell group and in combination group were decreased (P＜0.05).The apoptotic rate of renal epithelial cells in combination group was significantly lower than those in icariine and stem cell groups (P＜0.05).Conclusion:Icariine combined with embryonic stem cells has a protectice effect on the renal epithelial cells in the CIN rats,and its mechanism may be related to the inhibition of the related factors in the apoptosis pathways.
