Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Wuwei Shaji Granules by HPLC
10.6039/j.issn.1001-0408.2017.27.25
- VernacularTitle:HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量
- Author:
Jie GENG
1
;
Lihua CONG
;
Jinghui FAN
;
Chunyu ZHAO
;
Peifeng XUE
Author Information
1. 内蒙古医科大学药学院
- Keywords:
HPLC;
Wuwei shaji granules;
Liquiritin;
Glycyrrhizic acid;
Content determination
- From:
China Pharmacy
2017;28(27):3836-3838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.METHODS:HPLC method was adopted.The determination was performed on Thermo scientific ODS-2 Hypersil column with mobile phase consisted of acetonitrile-0.5% phosphoric acid (gradient elution) with flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 25 ℃.The sample size was 20 μL.RESULTS:The linear ranges of liquiritin and glycyrrhizic acid were 48-480 μg(r=0.999 9) and 90-900 μg(r=0.999 9),respectively.The limits of quantitation were 0.55,2.10 μg/mL,and the limits of detection were 0.16,0.62 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.08%-97.58% (RSD=0.93%,n=6)and 95.86%-99.89% (RSD=1.67%,n=6),respectively.CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the content determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.