Study on HPLC Fingerprint of Stir-baked Manis pentadactyla
10.6039/j.issn.1001-0408.2017.27.26
- VernacularTitle:炮山甲的HPLC指纹图谱研究
- Author:
Dan YAN
1
;
Yujie LIU
;
Meibian HU
;
He XIAO
;
Yongchuan LI
;
Chunjie WU
Author Information
1. 成都中医药大学药学院
- Keywords:
Stir-baked Manis pentadactyla;
HPLC;
Fingerprint;
Quality evaluation
- From:
China Pharmacy
2017;28(27):3839-3842
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprint of stir-baked Manis pentadactyla.METHODS:HPLC method was conducted.The determination was performed on Capcell Pak Mg Ⅱ S5 C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min.The detection wavelength was set at 275 nm,and column temperature was 30 ℃.The sample size was 10 μL.Using oxyphenylaminopropionic acid as reference,HPLC chromatograms of 11 batches of medicinal materials were determined.TCM Chromatogram Fingerprint Similarity Evaluation System (2004 A edition) was used for common peak identification and similarity evaluation.RESULTS:There were 23 common peaks in 11 batches of stir-baked M.pentadactyla,with similarity＞0.90.After validation,HPLC chromatograms of 11 batches of medicinal materials were in good agreement with control fingerprints.CONCLUSIONS:Established HPLC fingerprint can provide reference for the identification and quality evaluation of stir-baked M.pentadactyla.
