Simultaneous Determination of 4 Components in Modified Buyang Huanwu Decoction by RP-HPLC
10.6039/j.issn.1001-0408.2017.27.29
- VernacularTitle:RP-HPLC法同时测定加味补阳还五汤剂中4种成分的含量
- Author:
Zhongcheng WANG
1
;
Erli GU
;
Shenyan WANG
;
Fangfang WANG
;
Chong YU
;
Min ZHU
Author Information
1. 南通市第三人民医院
- Keywords:
RP-HPLC;
Modified buyang huanwu decoction;
Paeoniflorin;
Amygdalin;
Ferulic acid;
Ligustrazine;
Content determination
- From:
China Pharmacy
2017;28(27):3849-3852
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for simultaneous determination of paeoniflorin,amygdalin,ferulic acid and ligustrazine in Modified buying huanwu decoction.METHODS:RP-HPLC method was adopted.The determination was performed on YMC C18 column with the mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelengths were set at 320 nm (femlic acid),230 nm (paeoniflorin),207 nm (amygdalin),280 nm (ligustrazine).The column temperature was 30 ℃,and sample size was 10 μL.RESULTS:The linear ranges of paeoniflorin,amygdalin,femlic acid and ligustrazine were 0.191 2-1.912 μg/mL (r=0.999 6),0.117 4-1.174 μg/mL (r=0.999 6),0.011 5-0.115 μg/mL (r=0.999 8) and 0.001 66-0.016 6 μg/mL(r=0.999 7),respectively.The limits of quantitation were 1.912,1.174,0.115,0.016 6 μg/mL,and the limits of detection were 0.25,0.40,0.05,0.008 5 μg/mL,respectively.RSDs of precision,stability and reproducible tests were all lower than 2.0％.The recoveries were 96.9％-100.3％ (RSD=1.3％,n=6),95.1％-100.3％ (RSD=2.2％,n=6),95.3％-100.2％ (RSD=2.0％,n=6)and 97.0％-100.0％ (RSD=1.3％,n=6).CONCLUSIONS:The method is reliable,simple and accurate,and is suitable for simultaneous determination of paeoniflorin,amygdalin,ferulic acid and ligustrazine in Modified buyang huanwu decoction.
