Simultaneous Determination of (R,S)-goitrin and Chlorogenic Acid in Banchai Oral Liquid by Dual-wavelength HPLC
10.6039/j.issn.1001-0408.2017.27.30
- VernacularTitle:双波长HPLC法同时测定板柴口服液中(R,S)-告依春和绿原酸的含量
- Author:
Zhihui HUA
1
;
Dong LIU
;
Mingchun LI
Author Information
1. 郑州人民医院药学部临床药学科
- Keywords:
Banchai oral liquid;
(R,S)-goitrin;
Chlorogenic acid;
Content determination;
Dual-wavelength HPLC
- From:
China Pharmacy
2017;28(27):3853-3855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.METHODS:The dual-wavelength HPLC method was adopted.The determination was performed on Wondasil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (11∶89,V/V) at the flow rate of 1.0 mL/min.The detection wavelengths were 245 nm for (R,S)-goitrin and 327 nm for chlomgenic acid.The column temperature was 30 ℃,and injection volume was 10 μL.RESULTS:The linear ranges were 4.05-40.51 μg/mL for (R,S)-goitrin (r=0.999 9),29.41-294.05 μg/mL for chlorogenic acid (r=0.999 9).The limits of quantification were 3.32,2.45 ng,limits of detection were 1.00,0.74 ng.RSDs of precision,stability and reproducibility tests were lower than 1.0％;the recoveries were 98.46％-101.06％ (RSD=0.98％,n=9) and 98.18％-100.78％ (RSD=0.86％,n=9).CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.
