Simultaneous Determination of P-coumaric Acid and Ferulic Acid in Different Compatibility Proportions of the Couple of Sparganium stoloni and Curcuma aeruginosa by HPLC
10.6039/j.issn.1001-0408.2017.27.32
- VernacularTitle:HPLC法同时测定不同配伍比例三棱-莪术药对中P-香豆酸、阿魏酸的含量
- Author:
Yang LI
1
;
Fangru JI
;
Hongxiu PAN
;
Ximing ZONG
;
Yueying MIAO
;
Ming YANG
;
Lixin DING
Author Information
1. 佳木斯大学药学院/黑龙江省生物药制剂重点实验室
- Keywords:
HPLC;
Sparganium stoloni-Curcuma aeruginosa;
Couple;
P-coumaric acid;
Ferulic acid;
Content determination
- From:
China Pharmacy
2017;28(27):3859-3861
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of P-coumaric acid and ferulic acid in the couple of Sparganium stoloni and Curcuma aeruginosa,and to investigate rational proportion of the couple.METHODS:HPLC method was adopted.The determination was performed on Agilent 20RBAX XDB-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 266 nm,and the column temperature was 30 ℃.The sample size was 10 μL RESULTS:The linear ranges of P-coumaric acid and ferulic acid were 4.218 6-21.093 μg/mL(r=0.999 8),1.836 0-9.180 μg/mL(r=0.999 9),respectively.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 98.72％-100.30％ (RSD=0.18％,n=9),99.11％-100.45％ (RSD=0.46 ％,n =9).Among 4 compatibility proportions of the couple of S.stoloni and C.aeruginosa (1 ∶ 1,2 ∶ 1,1 ∶ 2,1 ∶ 0,m/m),the dis-solution rate of P-coumaric acid and ferulic acid were the highest when the ratio of S.stoloni-C.aeruginosa (m/m)was 2 ∶ 1.CON-CLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for content determination of P-coumaric acid and ferulic acid in the couple of S.stoloni and C.aeruginosa.when the proportion of the couple of S.stoloni and C.aeruginosa is 2∶1 and the contents of P-coumaric and ferulic acid are the highest,the contents of main etfective components are the highest.
