Comparative Study on HPLC Fingerprints of Taproot and Rhizome of Paeonia lactiflora
10.6039/j.issn.1001-0408.2017.27.34
- VernacularTitle:白芍药材主根与根茎的HPLC指纹图谱比较研究
- Author:
Zhiyong ZONG
1
;
Jinfeng ZHENG
;
Guangyi LU
;
Xianhong MI
;
Shuang SU
;
Jinghong WANG
Author Information
1. 中国中医科学院望京医院,北京100102
- Keywords:
Paenoia lactiflora;
Taproot;
Rhizome;
Fingerprint;
Similarity analysis
- From:
China Pharmacy
2017;28(27):3865-3867
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprints of taproot and rhizome of Paenoia lactiflora,and to compare the similarity and difference of them.METHODS:The determination was performed on Phenomenex C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 230 nm,and column temperature was 30 ℃.The sample size was 10 tL.Using paeoniflorin as reference,HPLC chromatograms of the taproot and rhizome of P lactiflora were established.Common peak identification and similarity evaluation were performed by using TCM Chromatogram Fingerprint Similarity Evaluation System (2012 edition).RESULTS:There were 9 common peaks in HPLC chromatograms of taproot and rhizome of P lactiflora.The similarity of taproot with rhizome of P lactiflora was higher than 0.9.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation of P lactiflora.The effective constituent of taproot and rhizome of P lactiflora are uniform but have small difference.
