The beneficial effect of mesenchymal stem cells transplantation in combination with interleukin-1 receptor antagonist on post-hepatectomy liver failure
10.3760/cma.j.issn.1007-8118.2017.09.012
- VernacularTitle:间充质干细胞移植联合白介素-1受体拮抗剂治疗肝切除后肝脏功能衰竭
- Author:
Jianfeng SANG
1
;
Hucheng MA
;
Xiaolei SHI
;
Tao HUANG
;
Dandan YI
;
Yitao DING
Author Information
1. 210008,南京大学医学院附属鼓楼医院肝胆外科
- Keywords:
Interleukin-1 receptor antagonist (IL-1 Ra);
Mesenchymal stem cells;
Stem cell transplantation;
Acute liver failure;
Apoptosis;
Regeneration
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(9):624-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the synergetic effect and possible mechanism of transplanting mesenchymal stem cells (MSCs) in combination with interleukin-1 receptor antagonist (IL-1Ra) on acute liver failure (ALF).Methods MSCs transplantation combined with IL-1Ra was used for a swine model of ALF induced by 85％ total hepatectomy.The living conditions,blood samples and survival time were recorded or collected for analysis of hepatic function.Liver injury histology was analyzed.Hepatic cell regeneration and apoptosis were studied by immunohistochemistry staining of Ki67 and TUNELassays respectively.The expression levels of AKT and NF-κB were analyzed by Western blotting.Results The difference on the survival time between the model group and combined therapy group was statistically significant (P ＜ 0.05).Combined therapy displayed improvement not only in the serum biochemical conditions but also in the serum inflammatory cytokines.Furthermore,the observed hepatic histopathological score was significantly less compared to model group.In addition,the combined therapy group significantly inhibited the liver cell apoptosis and increased hepatic cell regeneration.Finally,a significant increase in AKT expression and decrease of NF-κB expression (P ＜ 0.05) were observed,which was consistent with their important roles in liver regeneration.Conclusion The combined therapy displayed a synergistic effect on liver regeneration,by promoting restoration and reconstruction of ALF,through regulation of inflammation and apoptosis signaling network.
