The role of raffinose-low potassium dextran lung preservation solution with blood in pulmonary graft preservation
10.3760/cma.j.issn.0254-1785.2017.08.007
- VernacularTitle:含血棉子糖低钾右旋糖酐液在大鼠离体肺低温静态保存中的作用
- Author:
Guilong WANG
1
;
Zhenkun YANG
;
Zhiyi ZHOU
;
Zhiping WANG
;
Junli CAO
Author Information
1. 南京医科大学附属无锡市人民医院
- Keywords:
Rats;
Lung;
Whole blood;
Raffinose;
Low potassium dextran;
Perfusion;
Preservation
- From:
Chinese Journal of Organ Transplantation
2017;38(8):479-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential of a novel preservation solution,raffinose-low potassium dextran lung preservation solution with blood (BR-LPDS) in the preservation of the isolated lungs in rats,when compared to low-potassium dextran solution (LPDS).Methods Fifty donor lungs of Sprague-Dawley rats were randomly flushed with either R-LPDS (R-LPDS group,n =25) or RLPDS modified by the addition of blood (BR-LPDS group,n =25).After reperfusion,the double lungs were kept half inflate and then the pulmonary artery and bronchus were clamped.Then the grafts were flushed with and submerged in cold preservation solution at 4 C for 24 h.And they were excised for measurement of wet-to-dry ratio,as well as histologic examination to evaluate pulmonary edema,inflammation and cleaved caspase-3 expression at 0,2,4,5,6,8,10,12,14,16,24 h.Results As compared with the R-LPDS group,the mean wet-to-dry ratio of lungs in the BR-LPDS group was significantly reduced from 4 to 10 h after reperfusion.Upon histologic examination,less inflammatory cell aggregates and lower caspase-3 activity were seen in the lungs of the BR-LPDS group from 10 to 12 h and 10 to 14 h after reperfusion,respectively.Conclusion The addition of blood to the R-LPDS solution was shown to be more effective in reducing swelling,inflammation and caspase-3 expression of donor lungs than the R-LPDS solution.Further investigation is needed to evaluate lung preservation in BR-LPD solution as a viable option for transplant.
