Hypothermic machine perfusion promotes the regeneration of isolated liver

VernacularTitle:低温机器灌注保存对大鼠离体肝脏再生能力的影响
Author: Guifen WEI ¹ ; Jianhui LI ; Junjun JIA ; Yanfei ZHOU ; Wenfeng SONG ; Lin ZHOU
Author Information

1. 卫生和计划生育委员会多器官联合移植重点实验室
Keywords: Rat; Liver; Hypothermic machine preservation; Organ protection; Liver regeneration
From: Chinese Journal of Organ Transplantation 2017;38(8):484-488
CountryChina
Language:Chinese
Abstract: Objective To study the effects of different preservation methods on the isolated liver injury and regeneration.Methods Twelve Sprague-Dawley rats were randomly assigned to two groups:static cold storage (SCS) and hypothermic machine perfusion (HMP) (n =6 each).Histidinetryptophan-ketoglutarate solution (HTK) was used as preservation solution.The graft was preserved in HTK (4 C) for 6 h in SCS group,and that was perfused using HTK in HMP group.Liver tissue was obtained and fixed in 10％ neutral formalin for immunohistochemistry.The fresh liver tissue was collected and stored in-80℃ for RT-PCR and Western blotting analyses.Results Compared to SCS,HMP improved liver regeneration ability in terms of PCNA and ki67 detection and promoted cell proliferation in PCR levels (Cdc25A,cdk1,cdk6) and Western blotting levels (Cyelin D1,Cyclin E1).Conclusion HMP is superior to SCS in liver regeneration,and expected to be the ideal method for preservation of donor liver.