Effect of Kudiezi Injection on inflammation and TLR-4/NF-κB signal in cerebral ischemia reperfusion rats
10.7501/j.issn.1674-6376.2017.07.002
- VernacularTitle:苦碟子注射液对大鼠脑缺血再灌注损伤炎症及TLR-4/NF-κB信号的影响
- Author:
Xin ZHANG
1
;
han Shi GENG
;
Ping WANG
;
wei Peng ZHUANG
;
jun Yan ZHANG
Author Information
1. 天津中医药大学中药学院天津市中药药理重点实验室
- Keywords:
Kudiezi Injection;
cerebral ischemia reperfusion;
inflammation;
TLR-4/NF-κB signaling pathway
- From:
Drug Evaluation Research
2017;40(7):892-896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Kudiezi (KDZ) Injection on cerebral ischemia reperfusion injury in rats and to explore its protective mechanism.Methods The rat model of middle cerebral artery occlusion (MCAO) was established by modified suture method,and cerebral blood flow was monitored using laser Doppler flowmetry (LDF).Male SD rats were randomly divided into control group,model group,Kudiezi Injection high and low dose groups.After ischemia-reperfusion for 24 h,the neurological scores were evaluated.After anesthesia,the blood samples and brain tissues were collected,and the expression of inflammatory was detected by Elisa.Western blotting was used to detect the expression of TLR-4 and NF-κB protein.Results Behavioral scores showed that neural function defect was serious in model group compared with control group (P ＜ 0.01).In model group,cerebral index and cerebral infarction area were significantly higher than those of the control group;After KDZ intervention,the symptoms of neurological deficit was alleviated (P ＜ 0.01),the cerebral index and cerebral infarction area of mode were decreased,and the neuronal necrosis was reduced.Kudiezi Injection could significantly reduce the cerebral homogenate and serum levels of TNF-α (P ＜ 0.05) and increase IL-10 level (P ＜ 0.05).Westem blotting showed that Kudiezi Injection could reduce the expression of TLR-4 and NF-κB protein (P＜0.05).Conclusion Kudiezi Injection has protective effect on cerebral ischemia reperfusion rats.After ischemia-reperfusion,Kudiezi Injection could reduce the levels of TNF-α and raise IL-10.Its mechanism may be associated to the down-regulation of TLR-4/NF-κB signaling pathway.
