Gene silencing of indoleamine 2,3-dioxygenase 2 influences proliferation,migration and invasion of B16-BL6 melanoma cells
10.3969/j.issn.1000-4718.2017.09.014
- VernacularTitle:沉默吲哚胺2,3-双加氧酶2基因对黑色素瘤细胞B16-BL6增殖、迁移和侵袭的影响
- Author:
ping Xiao ZENG
1
;
Chu XIAO
;
xin Song YAN
;
ling Yan LIU
;
rong Hui ZHOU
;
yun Fang XU
;
Yi PAN
;
mei Hong WANG
Author Information
1. 南昌大学基础医学院
- Keywords:
Indoleamine 2,3-dioxygenase 2;
Melanoma;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2017;33(9):1619-1624
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of indoleamine 2,3-dioxygenase 2 (IDO2) silencing on proliferation,migration and invasion of B16-BL6 melanoma cells.METHODS:IDO2-siRNA was transfected into the B16-BL6 melanoma cells in vitro.The expression of IDO2 or IDOl at mRNA and protein levels was detected by real-time PCR and Western blot.Colony formation assay was performed to analyze the proliferation of IDO2-silencing tumor cells.The migration ability of B16-BL6 cells after silencing of IDO2 was measured by wound healing assay and Transwell cell migration assay.The invasion ability of the tumor cells was detected by Transwell cell invasion assay.RESULTS:IDO2-siRNA significantly down-regulated IDO2 expression in B16-BL6 melanoma cells,and did not affect IDO1 expression.Compared with control group,the colony formation ability,the migratory distance measured by wound healing assay,and the migration and the invasion cell numbers detected by Transwell assay all remarkably decreased in the IDO2-silencing cells.CONCLUSION:IDO2 silencing affects the proliferation,migration and invasion abilities of the R16-BL6 melanoma cells.
