Study on allergy of Astragaloside injection
10.7501/j.issn.1674-6376.2017.08.009
- VernacularTitle:黄芪甲苷注射液动物过敏性研究
- Author:
qin Bao JING
1
;
xiao Jin ZHANG
;
Wei KANG
;
Lei WANG
;
min Si XIONG
;
ping Xiu SHEN
Author Information
1. 天津药物研究院新药评价有限公司
- Keywords:
Astragaloside injection;
active systemic anaphylaxis (ASA);
passive cutaneous anaphylaxis (PCA)
- From:
Drug Evaluation Research
2017;40(8):1086-1089,1097
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the hypersusceptibility of Astragaloside injection on animal,and provide reference for clinical use with active systemic anaphylaxis (ASA),passive cutaneous anaphylaxis (PCA) and determination of serum sample titer.Methods ASA:Guinea pigs was ip with 0.4,1.6 mg/kg Astragaloside injection five times every other day.On the eleventh day after the last administration,the test substance was quickly injected to fore limb vein,and animal allergy symptoms were observed within 30 min.PCA:Astragaloside injection was ip injected to rats five times every other day and antiserum was collected.The antiserum was appropriately diluted,and sc injected to another group rats for passive sensitization.About 48 hours later,Astragaloside was quickly iv to rats,and the skin allergy was observed.Meanwhile,the antibody titer of the antiserum was determined.Results ASA:Astragaloside injection of 0.4,1.6 mg/kg in guinea pigs did not show any allergic reaction,that is,ASA was negative;PCA:Astragaloside injection of 0.5,2.0 mg/kg in rats did not show any allergic reaction,and Astragaloside specific antibodies were not determined in serum samples.That is,PCA was negative.Conclusion The results of ASA and PCA were negative in the experimental dose,and there was no specific antibody against Astragaloside in the serum prepared by PCA,which indicated that the possibility of hypersensitivity reaction was weak in clinical use.
