Effect of genistein on ammonia-induced NF-κB activation in astrocytes
10.3969/j.issn.1000-4718.2017.09.010
- VernacularTitle:染料木黄酮对氨诱导的星形胶质细胞NF-κB活化的影响
- Author:
Dan YANG
1
;
zhi Gui JIA
;
hui Ming CHU
;
liang Hong DAI
Author Information
1. 锦州医科大学附属第一医院
- Keywords:
Genistein;
Ammonia;
Astrocytes;
Nuclear factor-κB
- From:
Chinese Journal of Pathophysiology
2017;33(9):1598-1601
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of genistein on ammonia-induced nuclear factor-κB (NF-κB) activation and the underlying mechanism.METHODS:Primary astrocyte cultures were prepared and challenged with NH4Cl to establish a hyperammonemic model.The activation of ERK,Akt and NF-κB was examined by Western blot.RESULTS:AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt.Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002,genistein and AG1478.CONCLUSION:Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation,which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect.
