Effect of microRNA-7 knockdown on ConA-induced acute liver injury in mice
10.3969/j.issn.1000-4718.2017.09.018
- VernacularTitle:微小RNA-7敲减对ConA诱导的小鼠急性肝损伤的影响
- Author:
xu Dong YUE
1
;
juan Juan ZHAO
;
xiong Yi ZHANG
;
Tao DING
;
zi Hui CHEN
;
Lin XU
Author Information
1. 遵义医学院免疫学教研室暨贵州省生物治疗人才基地
- Keywords:
MicroRNA-7;
Acute liver injury;
CD4 + T cells
- From:
Chinese Journal of Pathophysiology
2017;33(9):1643-1647
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of microRNA-7 (miR-7) knockdown (KD) on concanavalin A (ConA)-induced acute liver injury (ALI) in mice.METHODS:Wild type (WT) mice and miR-7KD mice were received ConA (30 mg/kg) to induced acute liver injury model by intraperitoneal injection,and the morphological changes,liver weight and weight index were measured 48 h later.The pathological changes of the liver tissues were observed by HE staining.The levels of serum alanine aminotransferase (ALT),IL-4 and IFN-γ were detected by ELISA.The proportional changes of CD4 + T cells and the relative levels of IL-4 and IFN-γwere analyzed by flow cytometry.RESULTS:The color of the liver tissue became lighter,and the weight and weight index were changed significantly in miR-7KD mice compared with control group (P ＜ 0.05).HE staining showed that the inflammatory cell infiltration was increased in the liver of miR-7KD mice.Moreover,the level of serum ALT was significantly increased (P ＜ 0.05).The serum level of IFN-γelevated significantly (P ＜ 0.01),while the IL-4 levels decreased significantly (P ＜ 0.01) in the serum of miR-7KD mice.Furthermore,the proportion of CD4 + T cells and relative IFN-γcells increased obviously (P ＜ 0.01).CONCLUSION:miR-7 knockdown promotes the pathogenesis of the ConA-induced acute liver injury in mice.
