Expression of c-myc and c-ha-ras oncogenes in human bladder cancer.
- Author:
Choal Hee PARK
1
;
Min Ho SUH
;
Sung Choon LEE
Author Information
1. Department of Urology, Keimyung University. School of Medicine Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Bladder cancer;
C-myc;
C-Ha-ras;
Oncogene
- MeSH:
Blotting, Southern;
Carcinogenesis;
DNA;
Humans*;
Oncogenes*;
Point Mutation;
RNA;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1992;33(2):206-212
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to elucidate the c-myc and c-Ha-ras oncogenes expressions in human bladder cancers. we examined twelve bladder cancer tissues. two normal bladder tissues and two blood specimens with Southern. Northern and Slot blot hybridizations. In Southern blot all of the specimens showed 14 kilobase(Kb) c-myc band and 6.6kb c-Ha-ras band. No significant amplifications were observed in the levels of c-myc and c-Ha-ras DNA in cancer tissues. In Northern and slot blot, four bladder cancer tissues showed increased expression of c-myc RNA. No significant differences were observed in the levels of c-Ha-ras RNA expression between cancer tissues and normals. In conclusion. these findings support that quantitative increase in c-myc expression plays its role in the development of bladder cancers. Further studies about c-Ha-ras point mutation analysis and P21 ras protein analysis should be done to identify the role or c-Ha-ras in bladder tumorigenesis.
