Identification and establishment of sorting method for isolating CD11b+ myeloid cells in human hepatic metastases from colorectal cancer
10.3969/j.issn.1000-4718.2017.09.031
- VernacularTitle:人结直肠癌肝转移瘤中CD11b+髓系细胞分选方法的建立与鉴定
- Author:
xu Dong WANG
1
;
ting Ting HUO
;
wen Yao TIAN
;
Lei ZHAO
Author Information
1. 济南大学
- Keywords:
Colorectal liver metastases;
Flow cytometry;
Cell sorting;
CD11b+ myeloid cells;
Immunocytochemistry
- From:
Chinese Journal of Pathophysiology
2017;33(9):1723-1728
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for obtaining specific cells in solid tumor tissue by sorting of CD11b + myeloid cells in hepatic metastases from colorectal cancer.METHODS:Tumor tissues were prepared into single cell suspension by mechanical method combined with enzyme digestion,and then the CD11 b + myeloid cells were isolated by flow cytometry.The sorted cells were identified by immunocytochemistry.The viability and morphologiy of the sorted cells were evaluated by Giemsa and Typan blue staining.The cell purity was evaluated by flow cytometry.RESULTS:Sufficient numbers of CD11b+cells with high purity were isolated by sorting with flow cytometry from the single cell suspension prepared by mechanical and enzyme digestion.The purity of the cells was confirmed by statistical analysis (P ＜ 0.05).The positive rates of the cells before and after sorting were significantly different (P ＜0.01).The positive cells were verified by immunocytochemical method.Meanwhile,the sorted cells had complete morphology and good activity.CONCLUSION:The CD11b + myeloid cells in solid tumor tissue can be isolated by flow cytometry from the machine-enzyme digestion suspension with high purity,good activity and complete morphology.
