Correlation between gene polymorphism of protein tyrosine-phosphatase, non-receptor type22 and Graves disease in Qinghai Tibetan
10.3760/cma.j.issn.2095-4255.2017.09.003
- VernacularTitle:蛋白酪氨酸磷酸酶非受体型22(PTPN-22)基因多态性与青海藏族Graves病的相关性研究
- Author:
Shuqiong WANG
1
;
Yongli YAO
Author Information
1. 青海省人民医院内分泌科
- Keywords:
Protein tyrosine-phosphatase;
Gene polymorphism;
Graves disease;
Alleles
- From:
Chinese Journal of Endemiology
2017;36(9):635-638
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between gene polymorphism of protein tyrosinephosphatase,non-receptor type22 (PTPN-22) and Graves disease (GD) in Qinghai Tibetan.Methods One hundred and thirty Tibetan cases of GD were selected randomly from July 2012 to November 2016 in the People's Hospital of Qinghai Province;meanwhile,110 normal control cases were selected randomly fron the Qinghai Tibetan.All the subjects were non-related Tibetan residents of Qinghai nationality,who had no thyroid disease and other autoimmune diseases,and had no family history of autoimmune diseases.Then genotype and allele of PTPN-22 were detected by the method of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).Results The Hardy-Weinberg balance test was used to examine the representativeness of the control group and the GD group (P ＞ 0.05).The suggested samples have population representativeness and can be used in population genetics research.The distribution of PTPN-22 exon 14 (1 858C/T) genotype frequencies (CC,CT,TT) did not differ between normal control cases and GD in Qinghai Tibetan [95.45％ (105/110) vs 93.08％ (121/130),4.55％ (5/110) vs 6.92％ (9/130),0 (0/110) vs 0 (0/130),x2 =0.613,P ＞ 0.05].The distribution of PTPN-22 exon 14 (1 858C/T)allele frequencies (C,T) did not differ between nornal control cases and GD in Qinghai Tibetan [97.73％(215/220) vs 96.54％ (251/260),2.27％ (5/220) vs 3.46％ (9/260),x2 =0.015,P ＞ 0.05].Conclusion There is no significant relationship between the polymorphisms of PTPN-22 gene exon 14 (1 858C/T) and GD.
