Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity
10.3760/cma.j.issn.0254-5098.2017.10.001
- VernacularTitle:PDLIM4基因对胶质瘤预后及胶质瘤细胞放射敏感性的影响
- Author:
Ming LUO
1
;
Wangqing CAI
;
Kaishu LI
;
Jiahao LIU
;
Weiping XUE
;
Yimin LIU
Author Information
1. 中山大学孙逸仙纪念医院肿瘤放疗科
- Keywords:
PDLIM4;
Glioma;
Malignant degree;
Prognosis;
Radiation resistance
- From:
Chinese Journal of Radiological Medicine and Protection
2017;37(10):725-729,762
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PDLIM4 (PDZ and LIM domain 4) gene on the prognosis and radiosensitivity of glioma. Methods The differentially expressed genes were analyzed by bioinformatics technique using GSE53733 gene chip. The expression of PDLIM4 protein was detected by Western blot. The effects of PDLIM4 gene on glioma prognosis and glioma cell radiosensitivity were studied by using real-time fluorescence quantitative PCR, siRNA, MTT and cell flow cytometry assays. Results PDLIM4 gene had the most significant differential expression in the chip (logFC=1. 055897, P<0. 05). The PCR assay of 40 glioma cases in our hospital confirmed that expression of PDLIM4 showed obvious difference between high- and low-grade gliomas (t =4. 44, P <0. 05), which was correlated with the survival of patients (χ2 =5. 52, P<0. 05). Moreover, PDLIM4 gene was involved in radioresistance of glioma cells (t = 35.99, P < 0.05). Conclusions PDLIM4 gene expression level correlates with malignant degree and prognosis of glioma and also contributes to cell radioresistance.
