Effect of antisense locked nucleic acid blocking translation initiation region of c-myc exon 2 on apoptosis of hepatocellular carcinoma cells
10.3969/j.issn.1000-4718.2017.09.011
- VernacularTitle:反义锁核酸封闭c-myc第二外显子翻译起始区对肝癌细胞凋亡的影响
- Author:
bin Yi DENG
1
;
rong Shu XIAO
;
dan Gui XU
;
song Zan HUANG
Author Information
1. 右江民族医学院附属医院医学检验中心
- Keywords:
Hepatocellular carcinoma;
c-myc gene;
Antisense locked nucleic acid
- From:
Chinese Journal of Pathophysiology
2017;33(9):1602-1605
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of antisense locked nucleic acid (anti-LNA) blocking the translation initiation region of c-myc exon 2 on the viability and apoptosis of hepatocellular carcinoma cells.METHODS:The antiLNA that was complementary to the translation initiation region of c-myc exon 2 was designed,synthesized,and introduced into the HepG2 cells by cationic liposome-mediated transfection.The mRNA and protein levels of c-Myc in the cells were determined by RT-PCR and Western blot.The change of cell apoptosis was analyzed by flow cytometry,and the toxicity of anti-LNA to the cells was detected by MTT assay.RESULTS:Five days after transfection,the mRNA level of c-Myc in anti-LNA group was 0.335 ±0.016,and the protein level was 0.448 ± 0.037,significantly lower than those in control group (both P ＜ 0.05).The ratio of apoptotic cells in anti-LNA group was 32％ ±-6％,which was higher than that in control group (P ＜ 0.05).CONCLUSION:Antisense locked nucleic acid targeting at the translation initiation region of cmyc exon 2 shows strong inhibitory effects on the apoptosis of hepatocellular carcinoma cells.
