Effects of luteolin on invasion, migration and adhesion of human hepatocellular carcinoma HepG2 cells

VernacularTitle:木犀草素对人肝癌HepG2细胞株侵袭、迁移和黏附能力的影响
Author: yu Chun LI ¹ ; Qi WANG ; Shen SHEN ; xia Guo LI
Author Information

1. 天津医科大学国际医学院
Keywords: Luteolin; Hepatocellular carcinoma; Cell invasion; Cell migration; Cell adhesion; Epithelial-mesenchymal transition
From: Chinese Journal of Pathophysiology 2017;33(9):1606-1610
CountryChina
Language:Chinese
Abstract: AIM:To investigate the effects of luteolin on invasion,migration and adhesion of human hepatocelluar carcinoma HepG2 cells.METHODS:HepG2 cells were cultured and treated with luteolin at 10,20 and 40 μmol/L respectively.The invasion capability was examined by cell invasion assay.The migration ability was examined by wound healing assay.The adhesion capability was measured by adhesion assay.The protein levels of E-cadherin,N-cadherin,vimentin and Snai1 were determined by Western blot analysis.RESULTS:Luteolin inhibited the invasion,migration and adhesion ability of HepG2 cells in vitro in a dose-dependent manner.After treatment with luteolin,the expression of E-cadherin was increased significantly and the expression of N-cadherin,vimentin and Snai1 were decreased significantly.CONCLUSION:Luteolin inhibits the invasion,migration and adhesion ability of human hepatocelluar carcinoma HepG2 cells.The mechanism may be related to the regulatory effects of luteolin on epithelial-mesenchymal transition.