Effects of arsenic trioxide on T-bet/GATA3 signal pathway in MRL/lpr mice
10.3969/j.issn.1000-4718.2017.09.028
- VernacularTitle:三氧化二砷对MRL/lpr狼疮鼠T-bet/GATA3信号通路的影响
- Author:
fang Xiao ZHU
1
;
ye Hong HU
;
ji Yong LI
;
chun Xiao ZHU
;
Zhang ZHANG
;
Dan CHEN
Author Information
1. 温州医科大学附属第一医院风湿免疫科
- Keywords:
Arsenic trioxide;
MRL/lpr mice;
T-bet/GATA3 signal pathway;
IFN-γ;
IL-4
- From:
Chinese Journal of Pathophysiology
2017;33(9):1708-1712
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of arsenic trioxide (ATO) on T-bet/GATA3 signal pathway in MRL/lpr mice.METHODS:MRL/lpr mice and C57BL/6J mice at the age of 20 weeks were chosen and then divided in 2 different sub-groups,respectively.The mice in 2 sub-groups received ATO (0.4 mg · kg-1.d-1) and sodium chloride (NS,volume weight-determined) by intraperitoneal injection respectively for 2 months.Afterward,the spleens were isolated fron the MRL/lpr and C57BL/6J mice under pathogen-free condition and the suspensions were prepared.The mRNA level of T-bet,GATA3,IFN-γ,IL-4 and the mRNA ratio of T-bet/GATA3 were detected by RT-qPCR.The protein expression of T-bet and GATA3 was determined by Western blot.The serum levels of IFN-γ and IL-4 were measured by ELISA.RESULTS:The mRNA and protein levels of T-bet,IFN-γand the mRNA ratio of T-bet/GATA3 in NS group of MRL/lpr mice were higher than those in NS group of C57BL/6J mice (P ＜0.05).However,the GATA3 and IL-4 were lower in NS group of MRL/lpr mice in both mRNA and protein level (P ＜ 0.05).In MRL/lpr mice,the mRNA and protein levels of T-bet,IFN-γ and the mRNA ratio of T-bet/GATA3 were lower in ATO group compared with NS group (P ＜ 0.05),no difference was found in GATA3 and IL-4.No difference of the indexes mentioned above between ATO group and NS group in C57BL/6J mice was observed.CONCLUSION:ATO may affect the signaling pathway of T-bet/GATA3 to down-regulate the mRNA expression and the protein secretion of IFN-γ by decreasing the expression of T-bet in MRL/lpr mice.
