Study of application value of simple-rapid method in Escherichia coli identification
10.3969/j.issn.1673-4130.2017.20.017
- VernacularTitle:简略快速法在大肠埃希菌鉴定中的应用价值研究
- Author:
Xiaolong WANG
1
;
Siyu CHEN
;
Pingzong YUAN
;
Shuxia ZHANG
;
Yulin YANG
Author Information
1. 内江市第二人民医院检验科
- Keywords:
Escherichia coli;
biochemical identification;
rapid identification
- From:
International Journal of Laboratory Medicine
2017;38(20):2845-2846,2849
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the difference of simple-rapid identification method and automatic biochemical identification method in the identification of Escherichia coli .Methods The strains of Escherichia coli isolated from clinical samples were identi-fied by the simple-rapid method and automatic biochemical method .The consistency of result and the time of two methods were compared .Results Among 492 suspected strains ,248 strains were identified as Escherichia coli by simple-rapid method ,and other 244 strains were not .Meanwhile ,231 strains of these 248 Escherichia coli strains and 7 strains of 244 non Escherichia coli strains were identified as Escherichia coli by automatic biochemical method .The positive and negative predictive value of simple-rapid method were 93 .1% (231/248) and 97 .1% (237/244) .2 .5-7 .0 h [average(4 .12 ± 1 .08) h] were used to identify Escherichia coli by automatic biochemical method while0 .5-2 .0 h[average(1 .08 ± 0 .45) h] were used by simple-rapid method ,the difference was statistically significant(t= -40 .252 ,P<0 .001) .Conclusion The result of simple-rapid method is close to that of automatic bio-chemical identification method on Escherichia coli ,and simple-rapid method used less time .
