Rb1 protects human umbilical vein endothelial cells from hydrogen peroxide-induced senescence: involvement of caveolin-1
10.3969/j.issn.1000-4718.2017.09.002
- VernacularTitle:Rb1延缓人脐静脉内皮细胞早熟性衰老与caveolin-1表达的关系
- Author:
hui Ding LIU
1
;
Lin WU
;
jie Shu YU
;
Bin ZHOU
;
Min WANG
;
Yong LIU
;
shun Bao HAO
;
da Zhen ZHENG
;
xian Xiao QIAN
Author Information
1. 中山大学附属第三医院心血管内科
- Keywords:
Cell senescence;
Caveolin-1;
Human umbilical vein endothelial cells;
Ginsenoside Rb1
- From:
Chinese Journal of Pathophysiology
2017;33(9):1544-1550
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Endothelial cell senescence has been proposed to be involved in endothelial dysfunction and atherogenesis.This study aims to investigate the effects of ginsenoside Rb1,a major constituent of ginseng,on hydrogen peroxide (H2O2)-induced endothelial cell senescence,and to explore the expression and production of caveolin-1 in H2O2-induced premature senescence.METHODS:The senescence of primary human umbilical vein endothelial cells (HUVECs) was induced by H2O2 as judged by morphology inspection,senescence-associated β-galactosidase (SA-β-Gal) staining and cell cycle detection.The protein expression of caveolin-1 was determined by Western blot and confocal laser-scanning microscopy.RESULTS:Treatment of the HUVECs with H2O2 at 60 μmoL/L induced premature senescence,as judged by enlarged,flattened cell morphology,increased SA-β-Gal activity and sustained growth arrest.H2O2 effectively increased caveolin-1 level.Pretreatment of the HUVECs with Rb1 was found to reverse endothelial cell senescence,as witnessed by a significant decrease in senescent cell numbers and a decreased percentage of G0/G1 phase cells.Furthermore,Rb1 administration reversed the H2O2-increased protein level of caveolin-1.CONCLUSION:Ginsenoside Rb1 antagonizes H2O2-induced endothelial cell senescence through caveolin-1 modulation.
