Up-regulating expressions of proteins related to endoplasmic reticulum stress in rats with unilateral ureteral obstruction
- VernacularTitle:单侧输尿管梗阻的大鼠内质网应激相关蛋白表达上调
- Author:
hong Xiao TAO
1
;
Hao QIU
;
yun Xiao LYU
;
huan Shi MIAO
Author Information
1. 甘肃省第二人民医院
- Keywords:
endoplasmic reticulum stress;
renal interstitial fibrosis;
glucose regulated protein 78;
endoplasmic reticulum derived tran-scription factor;
cysteine aspartic proteinase
- From:
Basic & Clinical Medicine
2017;37(10):1412-1416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the endoplasmic reticulum stress (ERS) apoptosis pathway in unilateral ureteral obstruction (UUO) mechanism of renal interstitial fibrosis in rats.Methods The rats were randomly divided into con-trol group and model group,UUO group line ligation of the left ureter,three days 3,7 and 14 days HE and Masson staining were used to observe the renal pathological changes;Take blood retinal venous plexus,separat and determina-tion of serum blood urea nitrogen and creatinine;Western blot was used to find protein 78 (GRP78) glucose regula-tion,endoplasmic reticulum source sex transcription factor (CHOP) and apoptosis related proteins cysteine aspartic acid proteinase 3(caspase-3) and caspase-12 protein expression. Results Compared with control group,the visible UUO model group 1) expansion of renal tubule and renal interstitial fibrosis degree with the extension of ureteral ob-struction time and progressive increase;2) GRP78,CHOP,caspase-3 and caspase-12 protein expression in postoper-ative 3 d have to rise,as the obstruction prolonged,the protein expression more significantly(P<0.01).Conclusions Endoplasmic reticulum stress related trademark protein in UUO rat renal interstitial fibrosis is the increase in expres-sion may promote the early renal interstitial fibrosis and continuous progress.
