Preparation of Thrombin Aptamer Sensor Based on Host Guest Competition Model
10.11895/j.issn.0253-3820.170268
- VernacularTitle:基于主客体竞争模式的凝血酶适配体传感器的研究
- Author:
Lian Xue YAO
1
;
Dan Zha CHEN
;
Jing ZHANG
;
Feng Han CUI
;
Yan LIN
;
Nian HONG
;
Ling Ling HE
;
Rong De KONG
;
Hao FAN
;
Lin CHENG
Author Information
1. 江西中医药大学药学院
- Keywords:
β-Cyclodextrin;
Host-guest recognition;
Aptamer;
Thrombin
- From:
Chinese Journal of Analytical Chemistry
2017;45(10):1421-1426
- CountryChina
- Language:Chinese
-
Abstract:
A switch thrombin aptamer sensor was constructed based on the host-guest competition mode ofβ-cyclodextrin (β-CD ) . The aptamer that modified with ferrocene ( Fc ) at its terminal was fixed on the surface of the gold electrode via the host-guest recognization with β-CD. When thrombin was present, the configuration of aptamer transformed from vertical linear to "G-quadruplex" and far away from the electrode surface, which resulted in a decrease in the redox current of the aptamer probe and produce "Signal-off"effect. On the basis of this, a high sensitive detection of thrombin was made. The detection result indicated that the thrombin concentration had a consideration linear response to the signal of aptasensor between 5 . 0 × 10-13-5. 0 × 10-9 mol/L, and as low as 2. 0 × 10-13 mol/L thrombin had been detected. This method for thrombin detection showed a higher specificity compared to other protein molecules. Besides, the sensor was constructed easily and possessed excellent regeneration, which provided a significant platform for highly efficient real-time detection of thrombin in biological serum samples.
