A Magnetic Graphene and Paper-based Electrochemiluminescence Sandwich Immunoassay
10.11895/j.issn.0253-3820.170066
- VernacularTitle:一种基于磁性石墨烯的纸基电化学发光夹心免疫分析方法
- Author:
Yu CHEN
1
;
Jie WANG
;
Ming Zhong LIU
Author Information
1. 广州军区广州总医院医学实验科,广州510010
- Keywords:
Paper-based screen-printed electrode;
Magnetic graphene;
Electrochemiluminescence;
Immunoassay
- From:
Chinese Journal of Analytical Chemistry
2017;45(10):1455-1461
- CountryChina
- Language:Chinese
-
Abstract:
Based on the electrochemiluminescence and magnetic suspension immunoassay strategy, a new magnetic graphene and paper-based screen-printed electrode ( SPE ) electrochemiluminescence sandwich immunoassay was developed by combination of the unique physical and chemical properties of magnetic graphene and the advantages such as low cost and low-volume sample consumption of paper-based SPE. Taking human IgG as the model analyte, EDC/NHS method was used to couple capture antibody ( Ab1 ) and magnetic graphene, and direct labeling method was used to label signal antibody ( Ab2 ) with Ru NHS ester. Nonspecific adsorption was highly reduced by the magnetic suspension sandwich immunoassay strategy, and the concentration information of the analyte was obtained by paper-electrochemiluminescence detection. Experimental study of immobilization and labeling effect of Ab1 and Ab2 showed that the magnetic graphene not only improved the load of immune substances, but also promoted the electron transfer. The developed magnetic suspension and paper-based electrochemiluminescence immunoassay could realize the quantitative detection of IgG and had certain application prospect in low cost and rapid immune detection field.
